Use of proteases for gluten intolerance

摘要

The present technology relates to an enzyme composition. The enzyme composition may be used to treat gluten intolerant subjects, including suffering from non-Celiac gluten intolerance and/or non-Celiac gluten sensitivity. The enzyme composition may also be used to reduce gluten exposure in certain individuals. For example, the enzyme composition may also be used as a prophylactic to reduce exposure to gluten oligopeptides.

主权项

1. A method of treating gluten intolerance or reducing gluten exposure in a human subject comprising:
orally administering the subject with a therapeutically effective amount of an enzyme cocktail comprising a gluten degrading enzyme preparation isolated from Aspergillus oryzae with specificity for peptide substrate leucyl-glycyl-glycine (GDEP-LGG), papain, a semi-alkali protease and an enzyme preparation from Penicillium citrinum, wherein the enzyme cocktail degrades a gluten oligopeptide of SEQ ID NO:1 into peptide fragments of four amino acids or less at acidic conditions, and wherein the GDEP-LGG and papain are in a percent relative activity ratio of 80:20 to 20:80.