| 摘要 |
PURPOSE:To obtain a plasmid capable of preparing a large number of copies in a cell of bacterium, advantageously inserting different DNA into it, and its host. CONSTITUTION:A plasmid wherein different DNA are inserted into Xho I cite in a gene resistant to kanamycin. Namely, a transformed cell is selected by using chloramphenicol, and a cell sensitive to kanamycin is searched, to give plasmid pNR339 wherein different DNA fragments are cloned at Xho I cite. Escherichia coil K-12JC1569 is preferable as a host for plasmid pNR339. |
