BUFFERED WASH COMPOSITION, INSOLUBILIZING COMPOSITION, TEST KITS AND METHOD OF USE

摘要

BUFFERED WASH COMPOSITION, INSOLUBILIZING COMPOSITION, TEST KITS AND METHOD OF USE A buffered aqueous composition is useful simultaneously as a wash solution and a dye-providing composition in specific binding assays involving enzyme-labeled specific binding reagents. The wash composition includes a dye-providing composition, a buffer and an organic solvent having a certain molecular weight and water-solubility. Another useful composition includes a particulate substrate having avidin attached thereto, and a peroxidase reducing agent. Either composition can be provided in a diagnostic test kit, and can be used to detect a specific binding ligand in assays.