| 摘要 |
A method for enhancing the hybridization signal of a nucleic acid hybridization assay for the DNA or RNA of a target organism in a sample by adding the sample to a growth medium under conditions sufficient to allow any target organism in the sample to propagate, propagating the organism, if any, in the medium, for a time sufficient to allow the number of target organisms to reach a predetermined titer, removing trace minerals from the medium, adding a nucleic acid probe to the medium under stringency conditions sufficient to allow the probe to preferentially hybridize with the desired target organism, if any, to form hybridization products that emit an enhanced signal, and assaying the medium to detect the enhanced signal. The method is especially suited for use with RV medium in assays for Salmonella. |
