ELECTROCHEMICAL DETECTION OF DNA HYBRIDISATION

摘要

This invention relates to a method and apparatus for detecting DNA hybridisation and may be suitable for the detection of genetic defects. The invention provides a method of DNA hybridisation detection without the use of radioactive isotopes and includes the steps of: denaturing a specimen of DNA; contacting the specimen with an electrochemical electrode; measuring a reference polarographic signal characteristic of ssDNA; contacting the sample with an oligonucleotide probe under conditions where hybridisation may occur; removing any excess reagents; obtaining a polarographic signal from the electrode and comparing the signal with the reference signal.