发明名称 |
Method for the synthesis of ribonucleic acid (RNA) |
摘要 |
The invention relates to a method for the synthesis of ribonucleic acid (RNA) starting from deoxyribonucleic acid (DNA). In the method of the invention, a double stranded DNA molecule is cleaved with a restriction endonuclease so as to generate a free 3'-end. The resultant DNA molecule is then denatured and hybridized with a primer that anneals to the 3'-end of the denatured DNA. The primer used in the hybridization contains a T7 promoter sequence at its 5'-end. Following the hybridization step, the 3'-ends of the resultant hybrid DNA molecule are extended with DNA polymerase to generate a template suitable for T7 RNA polymerase mediated RNA synthesis.
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申请公布号 |
US5466586(A) |
申请公布日期 |
1995.11.14 |
申请号 |
US19940192756 |
申请日期 |
1994.02.07 |
申请人 |
AKZO NOBEL N.V. |
发明人 |
DAVEY, CHERYL;MALEK, LAWRENCE T.;LENS, PETER F.;WIELAARD, FRITS |
分类号 |
C12N15/09;C12P19/34;C12Q1/68;C12Q1/70;(IPC1-7):C07H21/00;C12N15/10 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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