Chemilumuniscent acridine derivatives and their use in luminescence immunoassays

摘要

A chemiluminescent acridinium derivative of the formula I in which R<1> is hydrogen, an alkyl, alkenyl or alkynyl radical having 1 to 10 carbon atoms, a benzyl or aryl group, R<2> and R<3> are hydrogen, an alkyl group having 1 to 4 carbon atoms, a substituted or unsubstituted amino group, a carboxyl, C1-C4-alkoxy, cyano or nitro group or halogen, R<4> represents a radical in which a sulphonamide group is bonded directly to the carbonyl group via the nitrogen or represents a thioalkyl or thioaryl radical of the formula II - S - X - R<5> (II) where X is a branched or unbranched aliphatic or aromatic group, which can also contain hetero atoms and R<5> is a reactive group which under mild conditions can selectively form a bond with amino, carboxyl, thiol or other functional groups in substances of biological interest, and A<(-)> is an anion impairing chemiluminescence; and its use in luminescence immunoassays.