发明名称 |
Methods for using mutant RNA polymerases with reduced discrimination between non-canonical and canonical nucleoside triphosphates |
摘要 |
A method for synthesizing a nucleic acid molecule comprising at least one non-canonical nucleoside triphosphate using a mutant polymerase having a reduced discrimination between canonical and non-canonical substrates is disclosed. The method comprises incubating a template nucleic acid in a reaction mixture comprising the mutant nucleic acid polymerase and the appropriate canonical and non-canonical nucleoside triphosphates which are desired substrates for the mutant nucleic acid polymerase. The present invention is also a method of determining the sequence of a nucleic acid molecule using the mutant polymerase to create a nucleic acid molecule comprising at least one non-canonical nucleoside triphosphate.
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申请公布号 |
US5849546(A) |
申请公布日期 |
1998.12.15 |
申请号 |
US19960713331 |
申请日期 |
1996.09.13 |
申请人 |
EPICENTRE TECHNOLOGIES CORPORATION;SOUSA, RUI |
发明人 |
SOUSA, RUI;JENDRISAK, JEROME J. |
分类号 |
C12N9/12;C12P19/34;C12Q1/68;(IPC1-7):C07H21/04 |
主分类号 |
C12N9/12 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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