发明名称 |
Recombinant antigen of Taenia solium metacestodes |
摘要 |
This invention is directed to an isolated DNA sequence encoding an antigen of Taenia solium metacestodes. A 10 kDa antigen of Taenia solium metacestodes (TSM) has been shown to be specific for immunodiagnosis of Neurocysticercosis (NCC), which is an important cause of neurological disease worldwide. This invention discloses a method of cloning a cDNA library encoding a 10 kDa protein from Taenia solium metacestodes. The cloned cDNA contained a 258 bp complete open reading frame, encoding an 86 amino acid protein with a calculated molecular weight of 9,582 Da. It showed 73% homology with a 10 kDa antigen of T. crassiceps. The recombinant protein was expressed bacterially as a fusion protein at a high level. A recombinant TSM antigen prepared according: to this invention is useful in detecting neurocysticerocosis disease. In immunoblot with purified recombinant protein, 97% of sera from active NCC showed strong reactivity while 14% of sera from chronic calcified NCC were weekly positive. In 180 sera from patients with other parasitic infections and from normal controls, it revealed 98% specificity.
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申请公布号 |
US6589752(B1) |
申请公布日期 |
2003.07.08 |
申请号 |
US20000676589 |
申请日期 |
2000.09.29 |
申请人 |
KONG YOON;CHUNG JOON-YOUNG;BAHK YOUNG YIL;KANG SHIN-YONG;CHO SEUNG-YULL |
发明人 |
KONG YOON;CHUNG JOON-YOUNG;BAHK YOUNG YIL;KANG SHIN-YONG;CHO SEUNG-YULL |
分类号 |
C07K14/435;C12N15/12;(IPC1-7):G01N33/53;C12P21/06;C07H19/00;C07K1/00 |
主分类号 |
C07K14/435 |
代理机构 |
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