СПОСОБ ИНТЕГРАЦИИ ГЕНОВ В СПЕЦИФИЧЕСКИХ САЙТАХ В КЛЕТКАХ МЛЕКОПИТАЮЩИХ ПОСРЕДСТВОМ ГОМОЛОГИЧНОЙ РЕКОМБИНАЦИИ И ВЕКТОРЫ ДЛЯ ОСУЩЕСТВЛЕНИЯ ЭТОГО СПОСОБА

摘要

A method for achieving site specific integration of a desired DNA at a target site in a mammalian cell via homologous recombination is described. This method provides for the reproducible selection of cell lines wherein a desired DNA is integrated at a predetermined transcriptionally active site previously marked with a marker plasmid. The method is particularly suitable for the production of mammalian cell lines which secrete mammalian proteins at high levels, in particular immunoglobulins. Novel vectors and vector combinations for use in the subject cloning method are also provided.