PCR KIT FOR CAPILLARY ELECTROPHORESIS

摘要

The present invention relates to a PCR kit for improving detection of particular nucleic acid motifs and for increasing the reliability of the results obtained.;More precisely, the invention proposes to use modified primers having particular characteristics making it possible, during electrophoresis treatment, to detect, on samples containing nucleic acids, the equivalent of a characteristic encoding of particular nucleic acid motifs, this encoding being such that it makes it possible to better detect the presence of these motifs.;The invention also proposes a method for detecting nucleic acid motifs of interest (for example STRs, mini-STRs or VNTRs) using such primers.;It advantageously finds—but is not limited to—application in the field of capillary electrophoresis.

主权项

1. PCR kit containing at least two primer pairs specific to a motif of interest to be identified on a nucleic acid, said pairs being adapted to generate, for said motif, at least two amplicons differentiable by their size and whose size differs by one base pair, by two base pairs, by three base pairs, or by four base pairs.