AUTOMATED DETERMINATION OF TOTAL BILE ACID IN SERUM AND REAGENT THEREFOR

摘要

PURPOSE:The concentration of bile acid as a substrate is determined by calculating the change rate in absorptivity of the characteristic absorption of diformazan which is formed by the reaction in the enzyme colorimetry. CONSTITUTION:The serum to be assayed is combined with (A) a buffer solution containing NADH2-dependent diaphorase, nicotinamide adenine dinucleotide and nitrobule tetrazolium and (B) another buffer solution containing 3alpha-hydroxysteroidaldehyde dehydrogenase. The total bile acid concentration in serum as the test specimen is determined from the change rate of the absorptivity in the characteristic absorption (540nm) of diformazan formed by the reaction.