PRODUCING METHOD FOR INTERLEUCIN-2

摘要

A fragment having -300 base pairs was obtained from a plasmid, PILM 7, cut by XbaI and PvuII. On the other hand, a fragment having - 4,500 base pairs was obtained from aplasmid, pNK2, treated with XbaI and PvuII. Both of the fragments were ligated and inserted into a proper strain to get pNKM 21. pNKM 21 was inserted into M 5248 strain to get KCTC 8258 P strain followed by culturing it to give a interleukin-2 contg. serine at 125-position instead of cystein.