METHOD FOR THE SEPARATION AND PURIFICATION OF T-PA

摘要

To separate and purify t-PA from an aqueous protein solution containing t-PA, an affinity chromatography is carried out on an insoluble carbohydrate which is substituted by a dye of the general formula <IMAGE> in which X = -Cl or <IMAGE> R1 = -H or -SO3<(-)> and R2 = -H, -OH or -OCH3, and the t-PA is extracted from a buffered solution at pH 5.5 to 8.5.