pH-RESPONSIVE PROTEOLYSIS PROBE

摘要

Provided is a tool exhibiting superior properties in the quantification of autophagic activity. The unimolecular FRET probe of the present invention comprises an acceptor consisting of a fluorescent protein undergoing enzymolysis in the lysosome or in the vacuole, and a donor having an amino acid sequence identical for at least 95% to the amino acid sequence represented as Sequence number 1.