FUSION PROTEIN FOR PROTEIN DETECTION, AND METHOD FOR DETECTING PROTEIN

摘要

A fusion protein for protein detection in which are fused: a protein domain including at least one among a C1 domain of protein G, a C2 domain of protein G, and a C3 domain of protein G; and a double mutant D153G/D330N of Escherichia coli alkaline phosphatase (BAP) in which the 153 amino acid residue Asp has been substituted by Gly and the 330 amino acid residue Asp has been substituted by Asn, a double mutant D153H/D330N of Escherichia coli alkaline phosphatase (BAP) in which the 153 amino acid residue Asp has been substituted by His and the 330 amino acid residue Asp has been substituted by Asn, or a double mutant K328R/D330N of Escherichia coli alkaline phosphatase (BAP) in which the 328 amino acid residue Lys has been substituted by Arg and the 330 amino acid residue Asp has been substituted by Asn.

主权项

1. A fusion protein for protein detection, wherein a protein domain, in which only three domains composed of a B domain of protein A, a C2 domain of protein G and a C3 domain of protein G are linked, is fused via the B domain of protein A to a C-terminal side or an N-terminal side of a double mutant D153G/D330N of Escherichia coli alkaline phosphatase (BAP) in which an amino acid residue Asp at position 153 has been substituted by Gly and an amino acid residue Asp at position 330 has been substituted by Asn, a double mutant D153H/D330N of Escherichia coli alkaline phosphatase (BAP) in which an amino acid residue Asp at position 153 has been substituted by His and an amino acid residue Asp at position 330 has been substituted by Asn, or a double mutant K328R/D330N of Escherichia coli alkaline phosphatase (BAP) in which an amino acid residue Lys at position 328 has been substituted by Arg and an amino acid residue Asp at position 330 has been substituted by Asn.