System and method for determining cell adequacy in a cytological analysis system

摘要

A cytological analysis test for 3D cell classification from a specimen. The method includes isolating and preserving a cell from the specimen and enriching the cell before embedding the enriched cell into an optical medium. The embedded cell is injected into a capillary tube where pressure is applied until the cell appears in a field of view of a pseudo-projection viewing subsystem to acquire a pseudo-projection image. The capillary tube rotates about a tube axis to provide a set of pseudo-projection images for each embedded cell which are reconstructed to produce a set of 3D cell reconstructions. Reference cells are classified and enumerated and a second cell classifier detects target cells. An adequacy classifier compares the number of reference cells against a threshold value of enumerated reference cells to determine specimen adequacy.

主权项

1. A cytological analysis test for 3D cell classification from a specimen comprising:
isolating and preserving at least one cell from the specimen; enriching the at least one cell; embedding the at least one enriched cell into an optical medium; injecting at least one embedded cell into a capillary tube; applying pressure to the at least one embedded cell until it appears in a field of view of a pseudo-projection viewing subsystem; acquiring at least one pseudo-projection image of at least embedded cell that is in the field of view using the pseudo-projection viewing subsystem; rotating the capillary tube about a tube axis to generate additional pseudo-projection images at different views using the pseudo-projection viewing subsystem to provide a set of pseudo-projection images for each embedded cell; reconstructing each embedded cell using data from the set of pseudo-projection images to produce a set of 3D cell reconstructions; operating a biological specimen classifier to detect reference cells, wherein the reference cells are identified and selected from the group comprising normal bronchial epithelial cells, metaplastic cells and columnar cells; enumerating the detected reference cells in the specimen; operating a second cell classifier with a predetermined single cell sensitivity value and predetermined single cell specificity value as characterized by a receiver operating curve (ROC) to detect target cells comprising abnormal lung cells; and operating an adequacy classifier to compare the number of reference cells against a threshold value of enumerated reference cells so as to determine specimen adequacy.