| 主权项 |
1. A plastic electrophoresis chip for separation and detection of DNA fragments comprising:
a substrate having top and bottom surfaces, further comprising an anode portion, a cathode portion, and a center portion between the anode and cathode portions, and a cover layer having top and bottom surfaces such that the top surface of said substrate layer is bonded with the bottom surface of said cover layer to form a plurality of non-intersecting microfluidic channels and a detection window, each nonintersecting microfluidic channel having an equivalent length of between 2-50 cm and a separation and detection region; and the length, widths and depths of each of the plurality of microfluidic channels is adjusted so that the resistances in each of the plurality of microfluidic channels is substantially equivalent and configured to detect DNA; and wherein, each non-intersecting microfluidic channel is in fluid communication with a first via located at said anode and a second via located at said cathode, said detection window comprised of plastic selected from the group consisting of polyethylene, poly(carbonate) and poly(acrylate) which essentially do not fluoresce light having a wavelength between 500 and 800 nm when excited at a wavelength between about 450 and 500 nm, and said detection window has a thickness of less than 2 mm, and overlaps in the detection region of each of said plurality of microfluidic channels. |
