RAPID WHOLE GENOME AMPLIFICATION

摘要

The present invention provides compositions and methods for rapidly amplifying target nucleic acid (e.g., using whole genome amplification) that allows small amounts of starting nucleic acid to be employed. In certain embodiments, the methods employ compositions that comprise: phi29 polymerase, exo-Klenow polymerase and/or Klenow polymerase, dNTPs, primers, and a buffering agent. In some embodiments, the target nucleic acid is amplified at a rate that would result in at least 1000-fold amplification in thirty minutes.

主权项

1. A method of amplifying target nucleic acid comprising:
a) providing a sample comprising: i) target nucleic acid, ii) phi29 polymerase, iii) exo-Klenow polymerase and/or Klenow polymerase, iv) dNTPs, v) primers, and vi) a buffering agent; and b) treating said sample under conditions such that said target nucleic acid is amplified thereby generating amplified target nucleic acid.