| 摘要 |
Recombinant Factor VIII can be produced in relatively large quantities on a cont inuous basis from mammalian cells in the absence of any animal-derived proteins such as albumin by culturing the cells in a protein free medium supplemented with polyol copolym ers, preferably in the presence of trace metals such as copper. In very preferred emb odiments, the medium includes a polyglycol known as Pluronic F-68, copper sulfate, ferrous sulfate/EDTA complex, and salts of trace metals such as manganese, molybdenum, silicon, lithium and chromium.
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