METHOD FOR ANALYSING N-GLYCANS IN COMPLEX BIOLOGICAL FLUIDS

摘要

A method for analysing N-glycans in complex biological fluids may comprise the steps of: adding protein to a sample including N-glycans, the protein including first marker glycans that have a different mass from the N-glycans; denaturing the protein in the sample after the step of adding the protein; separating glycans in the sample from the protein using an enzyme after the protein has denaturised; eluting glycans from the sample in which the protein is separated; obtaining a mass spectrum of the eluted glycans; and determining the efficiency or reaction of the enzyme based on the amount of first marker glycans in the mass spectrum. Using the method, pure N-glycans can be separated and refined from various complex biological fluids including serum, and data reliability can be improved by maximising efficiency and reproducibility in a short time using a well plate.