NUCLEIC ACID-FUNCTIONALIZED NANOPARTICLES

摘要

Provided herein are functionalized nanoparticle compositions and methods of using the same. The functionalized nanoparticles provided include a nuclease cleavage site and are, inter alia, useful for the formation of nanoparticle aggregates and detection of nuclease activity through nanoparticle aggregate formation.

主权项

1. A functionalized nanoparticle comprising a nanoparticle core and a nanoparticle coating, wherein said nanoparticle coating comprises:
a plurality of nucleic acid moieties bonded to said nanoparticle core, wherein each of said nucleic acid moieties comprises: (i) a first linker binding said nucleic acid moiety to said nanoparticle core; and (ii) a single-stranded nucleic acid sequence of about 50 or less nucleotides in length covalently attached to said first linker, wherein said single-stranded nucleic acid sequence comprises a nuclease cleavage site.