| 发明名称 |
METHOD FOR DETERMINING A PROTEIN-PROTEIN INTERACTION |
| 摘要 |
A method for determining an interaction between a first protein and a second protein comprises the steps of:
expressing in a cell or introducing into a cell
a first fusion protein comprising the first protein, a multimerizable protein, and a fluorescent protein, anda second fusion protein comprising the second protein and a multimerizable protein;detecting a fluorescent focus formed by an association between the first fusion protein and the second fusion protein in the cell; anddetermining an interaction between the first protein and the second protein according to the detection of the fluorescent focus. |
| 申请公布号 |
US2017122932(A1) |
申请公布日期 |
2017.05.04 |
| 申请号 |
US201515317587 |
申请日期 |
2015.06.10 |
| 申请人 |
MEDICAL & BIOLOGICAL LABORATORIES CO., LTD. |
发明人 |
WATANABE Taku;SHOJI Toru;MATSUZAWA Shun |
| 分类号 |
G01N33/50;G01N33/58;C07K14/435;G01N33/68 |
主分类号 |
G01N33/50 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method for determining an interaction between a first protein and a second protein, the method comprising the following steps (1) to (3):
(1) expressing in a cell or introducing into a cell
a first fusion protein comprising the first protein, a multimerizable protein, and a fluorescent protein, anda second fusion protein comprising the second protein and a multimerizable protein; (2) detecting a fluorescent focus formed by an association between the first fusion protein and the second fusion protein in the cell; and (3) determining an interaction between the first protein and the second protein according to the detection of the fluorescent focus. |
| 地址 |
Nagoya-shi, Aichi JP |
