QUANTITATIVE ANALYSIS OF TRANSGENIC PROTEINS

摘要

The invention relates to methods for quantitative multiplex analysis of complex protein samples from plants using mass spectroscopy. In some embodiments, the disclosure concerns methods for maintaining a transgenic plant variety, for example by analyzing generations of a transgenic plant variety for selective and sensitive quantitation of multiplexed transgenic proteins.

主权项

1. A high-throughput method of quantitating one or more protein of interest with known amino acid sequence in a plant-based sample, the method comprising:
(a) extracting proteins from a plant-based sample; (b) digesting proteins extracted from step (a) to obtain peptides; (c) separating the peptides in a single step; (d) determining a plural of signature peptides from the protein of interest with known amino acid sequence; (e) measuring the plural of signature peptides using high resolution accurate mass spectrometry (HRAM MS); and (f) quantitating the protein of interest with known amino acid sequence based on measurements of the signature peptides.