| 摘要 |
A test pack for detecting uric acid in blood comprises (a) a separately packed first buffered enzyme system comprising urate oxidase (uricase) and an inhibitor for enzymes having catalase-like activity and (b) a separately packed hydrogen peroxide detecting system. The blood sample is first contacted with (a) and after reaction, reagent (b) is added. The inhibitor may comprise an alkali metal azide or a cyanide. The buffer may have a pH of 8.5-10 (borate, glycine and tris(hydroxymethyl) aminomethane). A urate oxidase stabiliser (ethylene diamine, 1:2-propylenediamine, ethylene diamine tetracetic acid and other chelating agents for heavy metal ions) may also be present. Reagent (b) may comprise a chromogen the oxidation of which is catalysed by H2O2 in the presence of a peroxidative substance (o-tolidine, o- and p-tolidines, o-phenylenediamine, N:N/sv-dimethyl-p-phenylenediamine, o- and p-anisidines, dianisidine, o- and m-cresols, a - and b -naphthols, catechol, guaiacol, pyrogallol, 2:7-diaminofluorene, leuco-indophenols and gum guaiac), a peroxidative substance (peroxidase; normal whole blood, red blood cells, lyophilised whole blood; iodidemolybdate salts, molybdate salts alone; urohaemin, haemin and metallo-porphyrins, in combination with activating compounds including 2-aminobenzthiazole, pyridine, bipyridyl, bipyridylpyridine and nicotinic acid; iron tannate, ferrous ferro-cyanide and potassium chromic sulphate), a buffer of pH 4.4-5.5 (citrate, succinate, tris(hydroxymethyl) methylamine glutamate or malonate) if desired a further dye (tartrazine). p |
