METHOD FOR IDENTIFYING KIDNEY ALLOGRAFT RECIPIENTS AT RISK FOR CHRONIC INJURY

摘要

A method for identifying a renal allograft recipient at risk for chronic allograft damage or interstutial fibrosis and tubular atrophy (IF/TA) by comparing the transcription level of a preselected gene signature set with the transcription level of a comparison standard, and diagnosing the recipient as being at risk for chronic allograft damage if the transcription level of the preselected gene signature set is significantly higher than the transcription level of the comparison standard.

主权项

1. A method for identifying a renal allograft recipient at risk for chronic allograft damage or interstitial fibrosis and tubular atrophy (IF/TA) comprising the steps of:
(a) providing a biopsy specimen from the allograft; (b) providing an expression library as a comparison standard (c) isolating mRNA from the biopsy specimen; (d) synthesizing cDNA from the mRNA, producing a preselected gene signature set; (e) detecting the transcription level of a preselected gene signature set of step (d) (f) comparing the transcription level of the preselected gene signature set with the transcription level of a comparion standard, and (g) diagnosing the recipient as being at risk for chronic allograft damage or IF/TA if the transcription level of the preselected gene signature set is higher than the transcription level of the comparison standard.