Method for diagnosing alzheimer's disease (AD)

摘要

Disclosed is a method for diagnosing Alzheimer's disease (AD) wherein Aβ-specific antibodies in a biological sample of a person that is suspected of having AD are detected comprising the following steps: —contacting the sample with Aβ-aggregates or with particles having Aβ-aggregate like surfaces and allowing the Aβ-specific antibodies to bind to the Aβ-aggregates, and —detecting the Aβ-specific antibodies bound to the Aβ-aggregates by a single particle detection technique, preferably by fluorescence activated cell sorting (FACS); and wherein the amount of Aβ-specific antibodies detected is compared with the amount in a sample of known AD status.

主权项

1. A method for diagnosing Alzheimer's disease (AD), wherein Aβ-specific antibodies in a biological sample of a person that is suspected of haying AD are detected, the method comprising: contacting the sample with synthetic Aβ-aggregates and allowing the Aβ-specific antibodies to bind to the synthetic Aβ-aggregates, wherein the synthetic Aβ-aggregates are made by incubating Aβ at a pH from 2 to 9 for at least 20 minutes, and have a size of 50 nm to 15 μm, wherein the synthetic Aβaggregates do not comprise beads and the sample is not contacted with beads:
detecting the Aβ-specific antibodies bound to the synthetic Aβ-aggregates by a single particle detection technique selected from the group consisting of fluorescence-activated cell sorting and mass cytometry; and comparing an amount of Aβ-specific antibodies detected with an amount of Aβ-specific antibodies in a sample of known AD status.