CELL CULTURE SYSTEM

摘要

The embodiments of the invention described herein relate to systems and methods for culturing and/or maintaining intestinal cells, tissues and/or organoids in vitro. The cells, tissues and/or organoids cultured according to the methods and systems described herein can mimic or reproduce natural intestinal epithelial structures and behavior as well as support co-culture of intestinal microflora.

主权项

1. A method for maintaining the viability of intestinal cells and bacterial cells in co-culture comprising:
providing a fluidic device having:
a fluid channel connected to a fluid source, the fluid source supplying culture media to the fluid channel; anda membrane positioned within the channel between membrane support elements; introducing, to at least one surface of the membrane:intestinal epithelial cells; andviable bacterial cells; culturing the intestinal epithelial cells and bacterial cells for at least 48 hours; and wherein more than 95% of the intestinal epithelial cells are viable after the least 48 hours of culture.