METHOD FOR DIAGNOSING COLORECTAL CANCER FROM A HUMAN FECES SAMPLE BY QUANTITIVE PCR, PRIMERS AND KIT

摘要

The present invention relates to the field of detection of colorectal cancer (CRC). Specifically it relates to methods for the early detection, risk screening and monitoring of CRC and/or adenomatous polyps in a human subject based on the quantification of one or more 16S rDNA bacterial sequences in feces. It further relates to the use of said bacterial sequences as biomarkers of colorectal cancer and/or adenomatous polyps; to a kit comprising a reagent and instructions for the quantification of said bacterial sequences; and to nucleic acids for the quantification of said 16S rDNA bacterial sequences.

主权项

1. A method of screening for colorectal cancer (CRC) and/or adenomatous polyps in a human subject which comprises:
i. quantifying at least one 16S rDNA bacterial sequence selected from the list consisting of SEQ ID NO:1, SEQ ID NO:4, SEQ ID NO:7 and SEQ ID NO:10 or the complementary rRNA sequence thereof, from a feces sample from said subject; and ii. diagnosing, early detecting, determining recurrence, determining risk of developing, or predicting for CRC and/or polyps in a human subject, determining whether a colonoscopy should be performed in said human subject, determining prognosis wherein said human subject is a patient diagnosed with CRC and/or polyps, or guiding a therapy in a patient with CRC and/or polyps from the quantification levels of at least one of said sequences.