| 发明名称 |
SUMOylation Assay and Related Reagents |
| 摘要 |
Provided herein and methods and kits for identifying inhibitors of SUMOylation. The provided methods include contacting a candidate agent with (i) a SUMO-conjugating peptide comprising an N-terminal linker and a detectable tag, and (ii) a SUMO comprising a metal complex, under conditions that allow binding between the SUMO-conjugating peptide and the SUMO, and detecting the detectable tag thereby determining the level of binding between the SUMO-conjugating peptide and SUMO. A reduced level of binding as compared to the level of binding in the absence of the candidate agent indicating the agent inhibits SUMOylation. |
| 申请公布号 |
US2017096698(A1) |
申请公布日期 |
2017.04.06 |
| 申请号 |
US201615283682 |
申请日期 |
2016.10.03 |
| 申请人 |
The UAB Research Foundation |
发明人 |
Placzek William J.;Bjornsti Mary-Ann;Wright Christine M.;Whitaker Robert H. |
| 分类号 |
C12Q1/25;C12N9/00 |
主分类号 |
C12Q1/25 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method of identifying an inhibitor of SUMOylation comprising:
(a) contacting a candidate agent with (i) a SUMO-conjugating peptide comprising an N-terminal linker and a detectable tag, and (ii) a SUMO comprising a metal complex, under conditions that allow binding between the SUMO-conjugating peptide and the SUMO; and (b) detecting the detectable tag thereby determining the level of binding between the SUMO-conjugating peptide and SUMO; a reduced level of binding as compared to the level of binding in the absence of the candidate agent indicating the agent inhibits SUMOylation. |
| 地址 |
Birmingham AL US |
