CONTROL APPARATUS FOR DISPENSING SMALL PRECISE AMOUNTS OF LIQUID REAGENTS

摘要

A precision volumetric liquid dispensing instrument is disclosed that includes two pressure sensors and a fluid passageway with a defined volume portion in communication with the two sensors for receiving and distributing liquid in relatively small volumes. One of the pressure sensors is positioned to measure pressure at one portion of the defined volume portion of the fluid passageway and the other of the gas pressure sensors is positioned to measure gas pressure at a different portion of the defined volume portion of the passageway. At least one valve is in communication with the passageway for moving fluids into or out of the defined volume portion of the fluid passageway, and a processor carries out a step selected from the group consisting of (i) calculating the volume of the liquid based upon the measured pressure and (ii) metering a liquid into the defined volume portion of the fluid passageway until the measured pressure indicates that a desired volume of fluid is in the fluid passageway.

主权项

1. A method for transferring precise small volumes of liquids that is particularly useful for solid phase peptide synthesis (SPPS), the method comprising:
transferring a liquid to a defined volume portion of a fluid passageway; maintaining a gas with the liquid in the defined volume portion of the passageway; measuring the pressure in the defined volume portion; and calculating the volume of transferred liquid in the defined volume portion of the fluid passageway based upon the measured pressure.