发明名称 |
SPATIALLY RESOLVED LIGAND-RECEPTOR BINDING ASSAYS |
摘要 |
A method of detecting a ligand in a sample comprising the steps of: (a)
performing a ligand-receptor binding assay, wherein the ligand-receptor binding assay comprises combining a receptor attached to a solid support, a conjugate comprising a label, and a sample suspected of containing a ligand in a reaction vessel and allowing a reaction to occur; (b) acquiring an image to determine the location of the conjugate; (c) selecting at least one region of interest from the image; and (d) detecting a ligand in the sample. In particular, the detecting step comprises (i) selecting pixels from the at least one region of interest for analysis; (ii) calculating and recording the average and variance of the counts per pixel for the pixels selected in step (i); (iii) omitting pixels that have counts greater than or less than a specified variance; and (iv) calculating average counts per pixel of the remaining pixels. |
申请公布号 |
EP3151006(A1) |
申请公布日期 |
2017.04.05 |
申请号 |
EP20160198871 |
申请日期 |
2012.06.05 |
申请人 |
Abbott Laboratories |
发明人 |
QIAOQIAO, Ruan;SALDANA, Silvia C.;SKINNER, Joseph P.;TETIN, Sergey Y. |
分类号 |
G01N33/543;G01N21/64 |
主分类号 |
G01N33/543 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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