发明名称 |
METHODS OF SYNTHESIZING HETEROMULTIMERIC POLYPEPTIDES IN YEAST USING A HAPLOID MATING STRATEGY |
摘要 |
Methods are provided for the synthesis and secretion of recombinant hetero-multimeric proteins in mating competent yeast. A first expression vector is transformed into a first haploid cell; and a second expression vector is transformed into a second haploid cell. The transformed haploid cells, each individually synthesizing a non-identical polypeptide, are identified and then genetically crossed or fused. The resulting diploid strains are utilized to produce and secrete fully assembled and biologically functional hetero-multimeric protein. |
申请公布号 |
EP2330201(B1) |
申请公布日期 |
2017.04.05 |
申请号 |
EP20100183188 |
申请日期 |
2004.10.22 |
申请人 |
Keck Graduate Institute;ALDER BIOPHARMACEUTICALS, INC. |
发明人 |
CREGG, James M.;LATHAM, John;LITTON, Mark;SCHATZMANN, Randall;TOLSTORUKOV, IIya I. |
分类号 |
C12N15/81;C07K16/00;C07K16/28;C12N15/74;C12P21/04 |
主分类号 |
C12N15/81 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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