发明名称 |
Methods and compositions for targeted two-dimensional western blot analysis for early cancer detection and cancer diagnosis up to ten years in advance of clinical symptoms of malignant disease |
摘要 |
The present invention includes methods for detecting benign to malignant transformation of a cancer in a subject, comprising the steps of: collecting a sample from the subject prior to electrophoretic protein separation; activating electrophoretically separated ENOX2 transcript variants with an ENOX2 electron donor; and detecting the presence of the one or more activated ENOX2 transcript variants using a pan-ENOX2 detectable binding reagent, wherein the presence of one or more activated ENOX2 transcript variants in the sample is indicative of the malignant transformation of the cancer, whereby a 10 to 100 fold increase in detection sensitivity is obtained for the one or more activated ENOX2 transcript variants when compared to an equivalent non-activated ENOX2 transcript variant. |
申请公布号 |
US9612243(B1) |
申请公布日期 |
2017.04.04 |
申请号 |
US201615169205 |
申请日期 |
2016.05.31 |
申请人 |
MOR-NUCO ENTERPRISES, INC. |
发明人 |
Morre D. James |
分类号 |
G01N33/574 |
主分类号 |
G01N33/574 |
代理机构 |
Chalker Flores, LLP |
代理人 |
Flores Edwin S.;Chalker Flores, LLP |
主权项 |
1. A method for detecting one or more ENOX2 variants from a subject, comprising the steps of:
collecting a sample from the subject prior to electrophoretic protein separation; activating electrophoretically separated ENOX2 transcript variants with an ENOX2 electron donor; and detecting the presence of the one or more activated ENOX2 transcript variants using a pan-ENOX2 detectable binding reagent, wherein the presence of one or more activated ENOX2 transcript variants in the sample, whereby a 10 to 100 fold increase in detection sensitivity is obtained for the one or more activated ENOX2 transcript variants when compared to an equivalent non-activated ENOX2 transcript variant. |
地址 |
West Lafayette IN US |