RECOMBINANT IMMUNOGLOBULIN HEAVY CHAINS COMPRISING A SORTASE CONJUGATION LOOP AND CONJUGATES THEREOF

摘要

The present disclosure relates to a recombinant immunoglobulin heavy chain comprising a VH-domain a CH1 domain a hinge region down to the C-terminal most cysteine of the middle hinge region, a sortase conjugation loop, a CH2 domain and a CH3 domain, wherein the sortase conjugation loop a) consists of at least 16 amino acids located between the C-terminal most cysteine of the middle hinge region and the VFX1FPP (SEQ ID NO: 2; wherein X1 is L or I) consensus sequence at the N-terminus of an immunoglobulin heavy chain CH2-domain, b) comprises a sortase recognition motif consisting of the amino acid sequence LPX1TX2 (SEQ ID NO: 01), wherein X1 can be any amino acid residue and X2 is G or A, c) comprises N-terminal to the sequence of (b) at least two amino acids, d) comprises C-terminal to the sequence of (b) at least 6 amino acids, and e) wherein the amino acids C- and N-terminal to the sequence of (b) are not cysteine and its use in site-directed conjugation based on the enzyme sortase.