Labelled nucleotides

摘要

Nucleosides and nucleotides are disclosed that are linked to detectable labels via a cleavable linker group.

主权项

1. A method for determining the sequence of a target single-stranded polynucleotide, comprising monitoring the sequential incorporation of complementary nucleotides,
wherein the nucleotides each have a base that is linked to a detectable label vial a cleavable linker comprising an azido group, wherein each of the nucleotides has a ribose or deoxyribose sugar moiety and the ribose or deoxyribose sugar moiety comprises an azido protecting group attached via the 2′ or 3′ oxygen atom, and wherein the identity of each nucleotide incorporated is determined by detection of the label linked to the base, and subsequent removal of the label and the protecting group under a single set of conditions.