Composition for cryopreservation of cells and tissues

摘要

A composition for cryopreservation of cells and tissues of human and other animals in a safe manner without using toxic substances such as DMSO, as well as for freeze preserving or freeze-drying of foods and pharmaceuticals. In embodiments, ε-poly-L-lysine is reacted with succinic anhydride so that 60% or more of amino groups are blocked; and, thus obtained polymer compound is added to Dulbecco-modified eagle MEM culture medium (DMEM) on market sale to form a cryopreservation liquid. In embodiments for foods or pharmaceuticals, the ε-poly-L-lysine derivative was added by 0.5-10 wt % to curb freeze concentration.

主权项

1. A method for preserving cells or tissues, comprising: suspending or immersing the cells or tissues in a cryopreservative liquid; then, freezing the cryopreservative liquid, as well as the cells or tissues therein; and keeping them for a certain period and subsequently thawing them; wherein
said cryopreservative liquid is an aqueous solution comprising 1-50% by weight relative to the total weight of the cryopreservative liquid of a polyamine polymer compound comprised of a polymer of units having side-chain amino groups, said polymer of units being ε-poly-L-lysine; and 50-99 mol % of the amino groups, other than those forming amino-acid-to-amino-acid linkages, of said polymer compound are blocked with a carboxylic anhydride to form pendant moieties, each of which is linked to the main chain of the polymer via an amide linkage and has a free carboxylic acid group.