Method for producing cell concentrate

摘要

The present invention provides a method that allows the preparation of a cell concentrate in a short time without loss of cells and great damage on cells by simple operations. The present invention provides a method for producing a cell concentrate using an inside-out filtration system for processing a cell suspension, the system including: a cell suspension inlet port; a filtrate outlet port; a cell suspension outlet port; and a hollow fiber separation membrane interposed between the cell suspension inlet port and the cell suspension outlet port, wherein the hollow fiber separation membrane is provided with inner pores with an average pore size of 0.1 μm to 10 μm, and the quotient of the division of an initial filtrate flow rate by a linear velocity of the cell suspension flowing through the hollow fibers is 2.5 or less.

主权项

1. A method for producing a cell concentrate, the method comprising:
processing a human or animal cell suspension with an inside-out filtration system at a linear velocity of the cell suspension of 115 to 1020 cm/min, and at an initial filtrate flow rate, wherein the system comprises: a cell suspension inlet port; a filtrate outlet port; a cell suspension outlet port; and a hollow fiber separation membrane interposed between the cell suspension inlet port and the cell suspension outlet port, wherein the hollow fiber separation membrane has inner pores with an average pore size of from 0.1 μm to 0.5 μm, and a quotient of a division of the initial filtrate flow rate by the linear velocity of the cell suspension flowing through the hollow fibers is 2.5 or less.