Microorganism modified for the production of 1,3-propanediol

摘要

The invention relates to a modified microorganism for the production of PDO from a carbon substrate wherein the microorganism includes a three-step metabolic pathway including a first step of conversion of 2,4-dihydroxybutyrate (DHB) to obtain 2-oxo-4-hydroxybutyrate (OHB) by an enzyme having 2,4-DHB dehydrogenase activity, a second step of decarboxylation of the OHB to obtain 3-hydroxypropionaldehyde by an enzyme having 2-oxo-4-hydroxybutyrate decarboxylase activity, and a third step of reduction of the obtained 3-hydroxypropionaldehyde to obtain PDO with an enzyme having 3-hydroxypropionaldehyde reductase activity and the genes enabling the microorganism for the synthesis of DHB.

主权项

1. A modified microorganism for producing 1,3-propanediol (PDO) from a carbon substrate, the microorganism comprising:
a pathway for synthesis of 2,4-dihydroxybutyrate (DHB); and a three-step metabolic pathway comprising:
conversion of DHB to obtain 2-oxo-4-hydroxybutyrate (OHB) by an enzyme having 2,4-DHB dehydrogenase activity,decarboxylation of the OHB to obtain 3-hydroxypropionaldehyde by an enzyme having 2-oxo-4-hydroxybutyrate decarboxylase activity, andreduction of the obtained 3-hydroxypropionaldehyde to obtain PDO with an enzyme having 3-hydroxypropionaldehyde reductase activity, wherein the modified microorganism has been transformed by the introduction of at least three genes respectively encoding the enzyme having DHB dehydrogenase activity, the enzyme having 2-oxo-4-hydroxybutyrate decarboxylase activity, and the enzyme having 3-hydroxypropionaldehyde reductase activity.