MODIFYING MESSENGER RNA STABILITY IN PLANT TRANSFORMATIONS

摘要

Materials and methods for genome engineering through transient expression of a targeted nuclease are described herein. For example, the methods described herein can include introducing into a cell a messenger RNA (mRNA) that encodes a nuclease targeted to a selected sequence within the cell, where the stability of the mRNA is modified by the addition of untranslated regions (UTRs).

主权项

1. A nucleic acid comprising:
(a) a structural coding sequence encoding a rare-cutting endonuclease or a rare-cutting endonuclease subunit; and (b) a 5′ untranslated region (UTR), a 3′ UTR, or both a 5′ UTR and a 3′ UTR, wherein the 5′ UTR, 3′ UTR, or 5′ UTR and 3′ UTR are operably linked to the structural coding sequence.