发明名称 |
CULTURE METHOD FOR DIFFERENTIATING PRIMORDIAL GERM CELLS INTO FUNCTIONALLY MATURE OOCYTES |
摘要 |
The present invention addresses the problem of providing a method for differentiating primordial germ cells into functionally mature GV stage oocytes by in vitro culture. The present invention pertains to a method for differentiating primordial germ cells into functional GV stage oocytes in vitro including (a) a step for forming secondary follicles by culturing primordial germ cells and feeder cells adjacent to the primordial germ cells under conditions that eliminate the effects of estrogen or factors having a similar function to estrogen, (b) a step for partially cleaving the bonds between the granulosa cell layer and the thecal cell layer among the oocyte, granulosa cell layer, and thecal cell layer that constitute the formed secondary follicles, and (c) a step for differentiating the oocytes into functional GV stage oocytes by culturing the oocytes and granulosa cell layer that constitute the secondary follicles and the thecal cell layer in medium including a polymer compound. |
申请公布号 |
WO2017047799(A1) |
申请公布日期 |
2017.03.23 |
申请号 |
WO2016JP77574 |
申请日期 |
2016.09.16 |
申请人 |
TOKYO UNIVERSITY OF AGRICULTURE EDUCATIONAL CORPORATED;NATIONAL AGRICULTURE AND FOOD RESEARCH ORGANIZATION;KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION |
发明人 |
OBATA Yayoi;HIRAO Yuji;HAYASHI Katsuhiko |
分类号 |
C12N5/075;C12N5/0735;C12N15/09 |
主分类号 |
C12N5/075 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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