Method for purifying certain cell populations in blood or bone marrow by depleting others

摘要

A method for purifying and harvesting certain cell populations in blood or bone marrow by depleting at least one of red blood cells, granulocytes, or platelets from a sample comprising blood, bone marrow, or stromal vascular fraction cells separated from adipose tissue is disclosed. The apparatus comprises a sterile, single use rigid, self-supporting cartridge within which the automated depletion, purification and harvesting of target cell populations occurs and all components may be distributed.

主权项

1. A method for depleting at least one of red blood cells, granulocytes, or platelets from a sample comprising blood, bone marrow, or stromal vascular fraction cells separated from adipose tissue, the method comprising:
a. placing a rigid cartridge within a centrifuge, said rigid cartridge comprising a rigid chamber having an end that is fluidly connected to a valve system comprising at least one valve which is initially closed, a first rigid storage compartment, and a second rigid storage compartment; b. transferring said sample into said rigid chamber, said sample comprising platelets, plasma, cells of high density, and cells of low density; c. centrifuging said rigid cartridge such that said sample is urged toward said end by a first G force of at least 10 G and then by a second G force lower than said first G force; d. providing a first pathway for said cells of high density through said end and to said first rigid storage compartment; e. tracking a migration of said cells of high density through said end; and f. providing a second pathway for said cells of low density and an amount of platelets and plasma through said end and to said second rigid storage compartment.