Methods of Obtaining Pancreatic Endocrine Cells

摘要

The present invention relates to methods of producing pancreatic endocrine cells and uses of the cells obtained using the methods. The method utilises inhibitors or combinations of factors to provide increased quantities of endocrine material, for example for transplantation purposes.

主权项

1. A method for ex-vivo reprogramming comprising:
a) providing pancreatic cells to be reprogrammed, b) reprogramming the pancreatic cells, wherein the reprogramming comprises:
(i) treating the pancreatic cells with one or more transcription factors, the one or more transcription factors comprising PAX4, and(ii) inhibiting ARX expression and/or function c) thereby obtaining beta-like cells that express insulin mRNA.