APPARATUS, SYSTEM, AND METHOD FOR COLLECTING A TARGET MATERIAL

摘要

This disclosure is directed to an apparatus, system and method for retrieving a target material from a sample. An enrichment agent may be added to a vessel that contains the sample for positive selection, or, in other words, to select or aid in selecting the target material from amongst the remainder of the sample. The enrichment agent may be, for example, immunomagnetic beads, buoyant beads, high-density beads, chemicals to change the density of the target material, or the like.

主权项

1. A method comprising the steps of:
providing a primary vessel comprising an open end and a suspension comprising a target material; adding a positive enrichment reagent to the suspension for positive selection of the target material; inserting a device comprising a cannula into the open end of the primary vessel, wherein the device extends upwardly from the open end of the primary vessel; providing a processing vessel comprising
a first end comprising a resealable plug, andan inner cavity; mating the processing vessel with the device, wherein the cannula extends through the resealable plug of the processing vessel and accesses the inner cavity of processing vessel, and wherein the processing vessel extends upwardly from the device; adding a displacement fluid comprising a density greater than a density of the target material to the inner cavity of the processing vessel; and centrifuging the primary vessel, the device, the displacement fluid, and the processing vessel, wherein during centrifuging, the displacement fluid flows into the primary vessel via the device and displaces the target material from the primary vessel, which flows into the processing vessel through the device via the cannula.