| 摘要 |
The invention relates to a method for the in vitro production of arrangements of cell layers, wherein a first cavity (2.1) is provided, which is closed off from the surroundings of the first cavity except for a first inlet opening (4.1) and a first outlet opening (5.1) and which has a first wall (2.1.1) as a first cell substrate (6.1) and a second wall (2.1.2), which lies opposite and is separated from the first wall (2.1.1) by a first gap, as a second cell substrate (6.2), a free surface of a cell substrate (6.1, 6.2) to be populated with cells is oriented orthogonally to the gravitational force of the Earth, and cells are adhered to the cell substrate (6.1, 6.2) to be populated. The invention further relates to a method for maintaining the biological functionalities of the cell layers, to semi-finished products of a device for the in vitro production and cultivation of cell layers, and to a method for producing the device. |
