Measuring volume and constituents of cells

摘要

A method for determining a mean cell volume for a blood sample includes: illuminating the sample with incident light at a plurality of illumination wavelengths and obtaining a two-dimensional image of the sample at each of the plurality of illumination wavelengths; identifying a plurality of cells that appear in each of the images; for each one of the plurality of cells, determining an integrated optical density corresponding to each of the plurality of illumination wavelengths; for each one of the plurality of cells, determining a cell volume based on the integrated optical densities corresponding to each of the plurality of illumination wavelengths; and determining the mean cell volume for the blood sample from the cell volumes for each one of the plurality of cells.

主权项

1. A method for assessing an operating condition of a blood analysis device, the method comprising:
for each one of a plurality of cells in a control composition, using an electronic processor of the blood analysis device to:
determine at least one optical density value corresponding to a wavelength of illumination radiation incident on the cell;determine at least one cell volume value corresponding to the wavelength of the illumination radiation incident on the cell based on mean and maximum optical density values associated with the cell; anddetermine a hemoglobin content value by combining the at least one optical density value and the at least one cell volume value; using the electronic processor to determine a mean cell hemoglobin value for the control composition based on the hemoglobin content values of each one of the plurality of cells; using the electronic processor to compare a reference mean cell hemoglobin value for the control composition to the determined mean cell hemoglobin value for the control composition by determining a difference between the determined and reference mean cell hemoglobin values for the control composition; using the electronic processor to compare the difference to a threshold difference value; and re-calibrating the device if the difference is beyond the threshold difference value.