Thermal cycling system and method of use

摘要

A temperature cycling system (10, 110) is provided for repeatedly heating and cooling a reaction mixture (16). The system (10, 110) includes a first heater (27) and a second heater (28) each movable between a first orientation in which the first or second heater (27, 28) affects the temperature of the reaction mixture (16) and a second orientation in which the first or second heater (27, 28) does not substantially affect the temperature of the reaction mixture (16). During temperature cycling, the second heater (28) is in the second orientation when the first heater (27) is in the first orientation, and the second heater (28) is in the first orientation when the first heater (27) is in the second orientation.

主权项

1. A temperature cycling system for repeatedly heating and cooling a reaction mixture comprising:
a flexible reaction vessel configured to contain the reaction mixture and comprising a body having first and second portions coupled together, a first zone configured for receiving the first portion of the reaction vessel, the first zone comprising a first heater, the first zone movable between an open orientation in which the first heater affects the temperature of the reaction mixture contained within the first portion, and a closed orientation in which the reaction mixture is forced from the first portion and the first heater does not substantially affect the temperature of the reaction mixture, and a second zone configured for receiving the second portion of the reaction vessel, the second zone comprising a second heater, the second zone movable between an open orientation in which the second heater affects the temperature of the reaction mixture contained within the second portion, and a closed orientation in which the reaction mixture is forced from the second portion and the second heater does not substantially affect the temperature of reaction mixture; and wherein the flexible reaction vessel comprises a plurality of individual reaction vessels coupled together to form a row of reaction vessels, each individual reaction vessel including a body having first and second portions coupled together and a fitment having a channel fluidly connected to the first portion of a set of the individual reaction vessels, the fitment also having a port for receiving the reaction mixture, the port fluidly connected to the channel, the first zone is configured to receive a row of the first body portions, and the second zone is configured to receive a row of the second body portions.