Method for generating a microscope image and microscope

摘要

The invention relates to a method and a microscope for generating a microscopic image, wherein a) the sample is illuminated in each case by the microscope lens using the TIRF method; and b) the sample is illuminated in a structured fashion in different displacement positions of the structure. The sample light of the method according to a) and b) is detected in each case for generating an image of at least once sample region, wherein the sample images generated according to a) and b) are set off against one another, preferably multiplied, and the result is stored for generating a new sample image.

主权项

1. Method for generating a microscope image, comprising
(a) illuminating a specimen via a microscope objective lens by means of a total internal reflection fluorescence (TIRF) method and detecting specimen light to generate a first image of at least one specimen area, (b) subsequent or prior to generating said first image of said at least one specimen area by illuminating the specimen by means of the TIRF method, illuminating said specimen of said at least one specimen area in a structured way in different shift positions of the structure, and detecting specimen light when said specimen is illuminated at said at least one specimen area by structured light to generate multiple second images of said at least one specimen area, said second images being different from said first image, (c) separating orders of said secondary images, (d) filtering said orders of said secondary images by means of said first image by multiplication, and (e) combining said orders of said filtered secondary images by joint computation and displaying the combination.