METHOD AND APPARATUS FOR DETERMINING HEMOGLOBIN BASED PARAMETERS IN AN UNLYSED BLOOD SAMPLE

摘要

A method and apparatus for determining hemoglobin concentration is provided. A method aspect includes the steps of: a) depositing an unlysed, substantially undiluted blood sample into an analysis chamber adapted to quiescently hold the sample for analysis; b) imaging the sample in a region of the analysis chamber where the height of the chamber is no more than about twenty microns (20μ) or no less than about two microns (2μ), to produce image signals representative of the optical density of the imaged region; c) determining a sample representative optical density value using the image signals representative of the optical density of the imaged region; and d) determining the hemoglobin concentration of the sample using the sample representative optical density value.

主权项

1. A method for determining hemoglobin concentration of a blood sample, comprising:
depositing an unlysed, substantially undiluted blood sample into an analysis chamber adapted to quiescently hold the sample for analysis, the chamber defined by an interior surface of a first panel, and an interior surface of a second panel, wherein both panels are transparent, and which chamber has a height extending between the interior surface of the first panel and the interior surface of the second panel; using an analyzer having at least one processor, which at least one processor is in communication with at least one light source, at least one light detector, and with a memory device storing executable instructions, which instructions when executed cause the at least one processor to:
control the at least one light source to illuminate at least a portion of the sample residing within a region of the analysis chamber where the height of the chamber is no more than about twenty microns (20μ) and not less than about two microns (2μ), and receive image signals representative of an optical density (OD) of the illuminated sample portion from the at least one light detector, which illuminated sample portion includes non-red blood cell constituents;determine a sample representative OD value using the image signals representative of the OD of the sample within the illuminated sample portion; anddetermine the hemoglobin concentration of the sample using the sample representative optical density value.