| 发明名称 |
MUTANT PORES |
| 摘要 |
The invention relates to mutant forms of Msp. The invention also relates to polynucleotide characterisation using Msp. |
| 申请公布号 |
US2017058337(A1) |
申请公布日期 |
2017.03.02 |
| 申请号 |
US201515308206 |
申请日期 |
2015.05.01 |
| 申请人 |
Oxford Nanopore Technologies Ltd. Oxford Nanapore Technologies Limited |
发明人 |
Clarke James Anthony;Heron Andrew John;Jayasinghe Lakmal;Wallace Elizabeth Jayne |
| 分类号 |
C12Q1/68;C07K14/35 |
主分类号 |
C12Q1/68 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A mutant Msp monomer comprising a variant of the sequence shown in SEQ ID NO: 2, wherein the variant:
(a) does not comprise aspartic acid (D) at position 90; (b) does not comprise aspartic acid (D) at position 91; (c) comprises aspartic acid (D) or glutamic acid (E) at position 93; and (d) comprises one or more modifications which decrease the net negative charge of the inward facing amino acids in the cap forming region and/or the barrel forming region of the monomer. |
| 地址 |
Oxford GB |
